8R0S
Structure of reverse transcriptase from Cauliflower Mosaic Virus in complex with RNA/DNA hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-08 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.032340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.820, 140.520, 52.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.250 - 2.350 |
R-factor | 0.2168 |
Rwork | 0.214 |
R-free | 0.26310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 28172 | 4464 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.7 | |
CC(1/2) | 0.990 | 0.478 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 0.04M Citric acid, 0.06M Bis-Tris propane , 20% PEG 3350 |