8QYZ
Crystal structure of hiNES2 in complex with Xpo1 and RanGTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, B, E, G) | GTP-binding nuclear protein GSP1/CNR1 | polymer | 182 | 20672.9 | 4 | UniProt (P32835) Pfam (PF00071) | Saccharomyces cerevisiae S288C | |
| 2 | B, D, F, H (C, D, F, H) | Exportin-1 | polymer | 1048 | 120327.1 | 4 | UniProt (P30822) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Saccharomyces cerevisiae S288C | |
| 3 | I, J, K, L (I, J, K, L) | hiNES2 | polymer | 16 | 1750.0 | 4 | synthetic construct | ||
| 4 | M, P, S, V (A, B, E, G) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) | |||
| 5 | N, Q, T, W (A, B, E, G) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 6 | O, R, U, X (A, B, E, G) | ACETATE ION | non-polymer | 59.0 | 4 | Chemie (ACT) PubChem (175) | |||
| 7 | AA, BA, CA, DA, EA... (B, D, E, F, H...) | water | water | 18.0 | 209 | Chemie (HOH) |
Sequence modifications
A, B, E, G: 1 - 182 (UniProt: P32835)
C, D, F, H: 1 - 1084 (UniProt: P30822)
| PDB | External Database | Details |
|---|---|---|
| Leu 71 | Gln 71 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| - | Val 377 | deletion |
| - | Gln 378 | deletion |
| - | Arg 379 | deletion |
| - | Leu 380 | deletion |
| - | Pro 381 | deletion |
| - | Ala 382 | deletion |
| - | Thr 383 | deletion |
| - | Glu 384 | deletion |
| - | Met 385 | deletion |
| - | Ser 386 | deletion |
| - | Pro 387 | deletion |
| - | Leu 388 | deletion |
| - | Ile 389 | deletion |
| - | Gln 390 | deletion |
| - | Leu 391 | deletion |
| - | Ser 392 | deletion |
| - | Val 393 | deletion |
| - | Gly 394 | deletion |
| - | Ser 395 | deletion |
| - | Gln 396 | deletion |
| - | Ala 397 | deletion |
| - | Ile 398 | deletion |
| - | Ser 399 | deletion |
| - | Thr 400 | deletion |
| - | Gly 401 | deletion |
| - | Ser 402 | deletion |
| - | Gly 403 | deletion |
| - | Ala 404 | deletion |
| - | Leu 405 | deletion |
| - | Asn 406 | deletion |
| - | Pro 407 | deletion |
| - | Glu 408 | deletion |
| - | Tyr 409 | deletion |
| - | Met 410 | deletion |
| - | Lys 411 | deletion |
| - | Arg 412 | deletion |
| - | Phe 413 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 570999.7 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2426.1 | |
| All* | Total formula weight | 573425.9 |
