8QU4

NF-YB/C Heterodimer in Complex with a 13-mer NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker in an alternative binding pose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nuclear transcription factor Y subunit alpha	polymer	15	1748.2	1	UniProt (P23511)	Homo sapiens	CAAT box DNA-binding protein subunit A,Nuclear transcription factor Y subunit A,NF-YA
2	B (B)	Nuclear transcription factor Y subunit beta	polymer	95	10876.5	1	UniProt (P25208) Pfam (PF00808)	Homo sapiens (human)	CAAT box DNA-binding protein subunit B,Nuclear transcription factor Y subunit B,NF-YB
3	C (C)	Nuclear transcription factor Y subunit gamma	polymer	96	11258.2	1	UniProt (Q13952) Pfam (PF00125)	Homo sapiens (human)	CAAT box DNA-binding protein subunit C,Nuclear transcription factor Y subunit C,NF-YC,Transactivator HSM-1/2
4	D (C)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	E (C)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
6	F, G, H (A, B, C)	water	water		18.0	90	Chemie (HOH)

Sequence modifications

A: 270 - 282 (UniProt: P23511)

PDB	External Database	Details
Ace 269	-	acetylation
Nle 272	Tyr 272	engineered mutation
Nh2 283	-	amidation

B: 51 - 143 (UniProt: P25208)

PDB	External Database	Details
Gly 49	-	expression tag
Pro 50	-	expression tag

C: 27 - 120 (UniProt: Q13952)

PDB	External Database	Details
Gly 25	-	expression tag
Pro 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	23882.9
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	24067.1

*Water molecules are not included.