8QU4
NF-YB/C Heterodimer in Complex with a 13-mer NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker in an alternative binding pose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.450, 51.400, 71.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.820 - 1.380 |
| R-factor | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.19360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.754 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.820 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rpim | 0.011 | 0.300 |
| Number of reflections | 32648 | 3329 |
| <I/σ(I)> | 28.52 | 1.76 |
| Completeness [%] | 94.7 | 98.14 |
| Redundancy | 24.5 | 20.6 |
| CC(1/2) | 1.000 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 100 mM Morpheus buffer system 3 pH 9, 30% Morpheus precipitant mix 1, 100 mM Morpheus Carboxylic acids |
