8QOT

Structure of the mu opioid receptor bound to the antagonist nanobody NbE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mu-type opioid receptor	polymer	482	53994.2	1	UniProt (P42866) Pfam (PF00001) In PDB	Mus musculus (house mouse)
2	B	Nanobody E (NbE)	polymer	171	18458.7	1		Lama glama
3	H	NabFab HC	polymer	262	28220.5	1		synthetic construct
4	L	NabFab LC	polymer	238	25794.9	1		synthetic construct
5	K	Anti-Fab Nanobody	polymer	159	17414.4	1		Lama glama

Sequence modifications

A: -4 - 406 (UniProt: P42866)

PDB	External Database	Details
Met -25	-	initiating methionine
Lys -24	-	expression tag
Thr -23	-	expression tag
Ile -22	-	expression tag
Ile -21	-	expression tag
Ala -20	-	expression tag
Leu -19	-	expression tag
Ser -18	-	expression tag
Tyr -17	-	expression tag
Ile -16	-	expression tag
Phe -15	-	expression tag
Cys -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Phe -11	-	expression tag
Ala -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -3	Ser 3	conflict
Ala -2	Ser 4	conflict
Met -1	Ala 5	conflict
Glu 46	-	insertion
Asn 47	-	insertion
Leu 48	-	insertion
Tyr 49	-	insertion
Phe 50	-	insertion
Gln 51	-	insertion
Leu 359	-	insertion
Glu 360	-	insertion
Val 361	-	insertion
Leu 362	-	insertion
Phe 363	-	insertion
Gln 364	-	insertion
Gly 365	-	insertion
Pro 366	-	insertion
Ser 407	-	expression tag
Ser 408	-	expression tag
Leu 409	-	expression tag
Ala 410	-	expression tag
Glu 411	-	expression tag
Glu 412	-	expression tag
Asn 413	-	expression tag
Leu 414	-	expression tag
Tyr 415	-	expression tag
Phe 416	-	expression tag
Gln 417	-	expression tag
Ser 418	-	expression tag
Trp 419	-	expression tag
Ser 420	-	expression tag
His 421	-	expression tag
Pro 422	-	expression tag
Gln 423	-	expression tag
Phe 424	-	expression tag
Glu 425	-	expression tag
Lys 426	-	expression tag
Gly 427	-	expression tag
Gly 428	-	expression tag
Gly 429	-	expression tag
Ser 430	-	expression tag
Gly 431	-	expression tag
Gly 432	-	expression tag
Gly 433	-	expression tag
Ser 434	-	expression tag
Gly 435	-	expression tag
Gly 436	-	expression tag
Gly 437	-	expression tag
Ser 438	-	expression tag
Trp 439	-	expression tag
Ser 440	-	expression tag
His 441	-	expression tag
Pro 442	-	expression tag
Gln 443	-	expression tag
Phe 444	-	expression tag
Glu 445	-	expression tag
Lys 446	-	expression tag
Gly 447	-	expression tag
Ala 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	143882.6
All*	Total formula weight	143882.6

*Water molecules are not included.