8QOT
Structure of the mu opioid receptor bound to the antagonist nanobody NbE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Mu-type opioid receptor | polymer | 482 | 53994.2 | 1 | UniProt (P42866) Pfam (PF00001) | Mus musculus (house mouse) | |
| 2 | B (B) | Nanobody E (NbE) | polymer | 171 | 18458.7 | 1 | Lama glama | ||
| 3 | C (H) | NabFab HC | polymer | 262 | 28220.5 | 1 | synthetic construct | ||
| 4 | D (L) | NabFab LC | polymer | 238 | 25794.9 | 1 | synthetic construct | ||
| 5 | E (K) | Anti-Fab Nanobody | polymer | 159 | 17414.4 | 1 | Lama glama |
Sequence modifications
A: -4 - 406 (UniProt: P42866)
| PDB | External Database | Details |
|---|---|---|
| Met -25 | - | initiating methionine |
| Lys -24 | - | expression tag |
| Thr -23 | - | expression tag |
| Ile -22 | - | expression tag |
| Ile -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Tyr -17 | - | expression tag |
| Ile -16 | - | expression tag |
| Phe -15 | - | expression tag |
| Cys -14 | - | expression tag |
| Leu -13 | - | expression tag |
| Val -12 | - | expression tag |
| Phe -11 | - | expression tag |
| Ala -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -3 | Ser 3 | conflict |
| Ala -2 | Ser 4 | conflict |
| Met -1 | Ala 5 | conflict |
| Glu 46 | - | insertion |
| Asn 47 | - | insertion |
| Leu 48 | - | insertion |
| Tyr 49 | - | insertion |
| Phe 50 | - | insertion |
| Gln 51 | - | insertion |
| Leu 359 | - | insertion |
| Glu 360 | - | insertion |
| Val 361 | - | insertion |
| Leu 362 | - | insertion |
| Phe 363 | - | insertion |
| Gln 364 | - | insertion |
| Gly 365 | - | insertion |
| Pro 366 | - | insertion |
| Ser 407 | - | expression tag |
| Ser 408 | - | expression tag |
| Leu 409 | - | expression tag |
| Ala 410 | - | expression tag |
| Glu 411 | - | expression tag |
| Glu 412 | - | expression tag |
| Asn 413 | - | expression tag |
| Leu 414 | - | expression tag |
| Tyr 415 | - | expression tag |
| Phe 416 | - | expression tag |
| Gln 417 | - | expression tag |
| Ser 418 | - | expression tag |
| Trp 419 | - | expression tag |
| Ser 420 | - | expression tag |
| His 421 | - | expression tag |
| Pro 422 | - | expression tag |
| Gln 423 | - | expression tag |
| Phe 424 | - | expression tag |
| Glu 425 | - | expression tag |
| Lys 426 | - | expression tag |
| Gly 427 | - | expression tag |
| Gly 428 | - | expression tag |
| Gly 429 | - | expression tag |
| Ser 430 | - | expression tag |
| Gly 431 | - | expression tag |
| Gly 432 | - | expression tag |
| Gly 433 | - | expression tag |
| Ser 434 | - | expression tag |
| Gly 435 | - | expression tag |
| Gly 436 | - | expression tag |
| Gly 437 | - | expression tag |
| Ser 438 | - | expression tag |
| Trp 439 | - | expression tag |
| Ser 440 | - | expression tag |
| His 441 | - | expression tag |
| Pro 442 | - | expression tag |
| Gln 443 | - | expression tag |
| Phe 444 | - | expression tag |
| Glu 445 | - | expression tag |
| Lys 446 | - | expression tag |
| Gly 447 | - | expression tag |
| Ala 448 | - | expression tag |
| His 449 | - | expression tag |
| His 450 | - | expression tag |
| His 451 | - | expression tag |
| His 452 | - | expression tag |
| His 453 | - | expression tag |
| His 454 | - | expression tag |
| His 455 | - | expression tag |
| His 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 143882.6 | |
| All* | Total formula weight | 143882.6 |
