8QMN

[FeFe]-hydrogenase maturase HydE from T. maritima - dialysis experiment - empty structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	[FeFe] hydrogenase maturase subunit HydE	polymer	358	40991.3	1	UniProt (Q9X0Z6) Pfam (PF04055) In PDB	Thermotoga maritima MSB8
2	A	3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE	non-polymer		614.9	1	Chemie (CPS)
3	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
4	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
5	A	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
6		water	water		18.0	482	Chemie (HOH)

Sequence modifications

A: 2 - 348 (UniProt: Q9X0Z6)

PDB	External Database	Details
Met -9	-	initiating methionine
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Ile 291	Met 291	engineered mutation
Ser 311	Cys 311	engineered mutation
Ser 319	Cys 319	engineered mutation
Ser 322	Cys 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40991.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1492.7
All*	Total formula weight	42484.1

*Water molecules are not included.