8QMN
[FeFe]-hydrogenase maturase HydE from T. maritima - dialysis experiment - empty structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.740, 83.630, 70.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.910 - 1.480 |
| R-factor | 0.13528 |
| Rwork | 0.133 |
| R-free | 0.17695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.812 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 62674 | 8039 |
| <I/σ(I)> | 13.12 | |
| Completeness [%] | 98.5 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.999 | 0.257 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, Tris pH 8, LiSO4 |
