8QH5

CryoEM structure of UVSSA(VHS)-CSA-DDB1-DDA1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	UV-stimulated scaffold protein A	polymer	729	82923.2	1	UniProt (Q2YD98) Pfam (PF09740) Pfam (PF20867)	Homo sapiens (human)
2	B (A)	DNA excision repair protein ERCC-8	polymer	408	45465.6	1	UniProt (Q13216) Pfam (PF00400)	Homo sapiens (human)	Cockayne syndrome WD repeat protein CSA
3	C (B)	DNA damage-binding protein 1	polymer	1160	129298.9	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF23726) Pfam (PF03178)	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
4	D (C)	DET1- and DDB1-associated protein 1	polymer	152	16997.6	1	UniProt (Q9BW61) Pfam (PF10172)	Homo sapiens (human)	Placenta cross-immune reaction antigen 1,PCIA-1

Sequence modifications

D: 1 - 709 (UniProt: Q2YD98)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

A: 1 - 396 (UniProt: Q13216)

PDB	External Database	Details
Gly 397	-	expression tag
Thr 398	-	expression tag
Ser 399	-	expression tag
Ala 400	-	expression tag
Trp 401	-	expression tag
Ser 402	-	expression tag
His 403	-	expression tag
Pro 404	-	expression tag
Gln 405	-	expression tag
Phe 406	-	expression tag
Glu 407	-	expression tag
Lys 408	-	expression tag

B: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

C: 1 - 102 (UniProt: Q9BW61)

PDB	External Database	Details
Asp 103	-	expression tag
Val 104	-	expression tag
Leu 105	-	expression tag
Phe 106	-	expression tag
Gln 107	-	expression tag
Gly 108	-	expression tag
Pro 109	-	expression tag
Gly 110	-	expression tag
Ala 111	-	expression tag
Trp 112	-	expression tag
Ser 113	-	expression tag
His 114	-	expression tag
Pro 115	-	expression tag
Gln 116	-	expression tag
Phe 117	-	expression tag
Glu 118	-	expression tag
Lys 119	-	expression tag
Gly 120	-	expression tag
Gly 121	-	expression tag
Gly 122	-	expression tag
Ser 123	-	expression tag
Gly 124	-	expression tag
Gly 125	-	expression tag
Gly 126	-	expression tag
Ser 127	-	expression tag
Gly 128	-	expression tag
Gly 129	-	expression tag
Gly 130	-	expression tag
Ser 131	-	expression tag
Trp 132	-	expression tag
Ser 133	-	expression tag
His 134	-	expression tag
Pro 135	-	expression tag
Gln 136	-	expression tag
Phe 137	-	expression tag
Glu 138	-	expression tag
Lys 139	-	expression tag
Gly 140	-	expression tag
Ala 141	-	expression tag
Ser 142	-	expression tag
Gly 143	-	expression tag
Glu 144	-	expression tag
Asp 145	-	expression tag
Tyr 146	-	expression tag
Lys 147	-	expression tag
Asp 148	-	expression tag
Asp 149	-	expression tag
Asp 150	-	expression tag
Asp 151	-	expression tag
Lys 152	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	274685.3
All*	Total formula weight	274685.3

*Water molecules are not included.