8QFC

UFL1 E3 ligase bound 60S ribosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	60S ribosomal protein L10a	polymer	217	24879.4	1	UniProt (P62906) Pfam (PF00687) In PDB	Homo sapiens (human)	CSA-19,Large ribosomal subunit protein uL1,Neural precursor cell expressed developmentally down-regulated protein 6,NEDD-6
2	B	E3 UFM1-protein ligase 1	polymer	809	91591.2	1	UniProt (O94874) Pfam (PF09743) In PDB	Homo sapiens (human)	E3 UFM1-protein transferase 1,Multiple alpha-helix protein located at ER,Novel LZAP-binding protein,Regulator of C53/LZAP and DDRGK1
3	C	CDK5 regulatory subunit-associated protein 3	polymer	511	57458.9	1	UniProt (Q96JB5) Pfam (PF05600) In PDB	Homo sapiens (human)	CDK5 activator-binding protein C53,LXXLL/leucine-zipper-containing ARF-binding protein,Protein HSF-27
4	D	DDRGK domain-containing protein 1	polymer	303	34644.4	1	UniProt (Q96HY6) Pfam (PF09756) In PDB	Homo sapiens (human)	Dashurin,UFM1-binding and PCI domain-containing protein 1
5	E	Ubiquitin-fold modifier 1	polymer	87	9236.6	1	UniProt (A0A8C2YGR4) Pfam (PF03671) UniProt (by SIFTS) (P61960) In PDB	Homo sapiens (human)

Sequence modifications

B: 1 - 794 (UniProt: O94874)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

C: 1 - 506 (UniProt: Q96JB5)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag

D: 29 - 314 (UniProt: Q96HY6)

PDB	External Database	Details
Met 12	-	initiating methionine
Trp 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Pro 16	-	expression tag
Gln 17	-	expression tag
Phe 18	-	expression tag
Glu 19	-	expression tag
Lys 20	-	expression tag
Leu 21	-	expression tag
Glu 22	-	expression tag
Val 23	-	expression tag
Leu 24	-	expression tag
Phe 25	-	expression tag
Gln 26	-	expression tag
Gly 27	-	expression tag
Pro 28	-	expression tag

E: -3 - 83 (UniProt: A0A8C2YGR4)

PDB	External Database	Details
Pro -2	Ser 113	conflict
Ser 5	Thr 120	conflict
Ile 8	Val 123	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	217810.7
All*	Total formula weight	217810.7

*Water molecules are not included.