8QBL

Retron-Eco1 filament with inactive effector (E106A, 2 segments)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, I, K, P, U...	Retron Ec86 reverse transcriptase	polymer	349	39984.4	6	UniProt (P23070) Pfam (PF00078) In PDB	Escherichia coli BL21(DE3)
2	C, M, O, W, b...	Retron-Eco1-msr	polymer	82	26199.4	6	GenBank (296142109)	Escherichia coli BL21(DE3)
3	D, N, S, X, Y...	Retron-Eco1-A2	polymer	14	4541.8	6		Escherichia coli BL21(DE3)
4	G, E, F, H, T	Retron Ec86 putative ribosyltransferase/DNA-binding protein	polymer	307	35480.2	5	UniProt (P0DV88) In PDB	Escherichia coli BL21(DE3)	ORF223
5	L, B, J, Q, V...	Retron-Eco1 msDNA	polymer	85	26320.9	6	GenBank (296142109)	Escherichia coli BL21(DE3)
6	A, L, U, Q, a	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	E, F, T	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)

Sequence modifications

A, I, K, P, U, Z: 1 - 320 (UniProt: P23070)

PDB	External Database	Details
Gly 321	-	expression tag
Ser 322	-	expression tag
Glu 323	-	expression tag
Phe 324	-	expression tag
Glu 325	-	expression tag
Leu 326	-	expression tag
Glu 327	-	expression tag
Asn 328	-	expression tag
Leu 329	-	expression tag
Tyr 330	-	expression tag
Phe 331	-	expression tag
Gln 332	-	expression tag
Gly 333	-	expression tag
Glu 334	-	expression tag
Leu 335	-	expression tag
Arg 336	-	expression tag
Arg 337	-	expression tag
Gln 338	-	expression tag
Ala 339	-	expression tag
Ser 340	-	expression tag
Ala 341	-	expression tag
Leu 342	-	expression tag
Glu 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

G, E, F, H, T: 1 - 307 (UniProt: P0DV88)

PDB	External Database	Details
Ala 106	Glu 106	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	29
	Total formula weight	759679.7
Non-Polymers*	Number of molecules	9
	Total formula weight	2775.2
All*	Total formula weight	762454.9

*Water molecules are not included.