8Q7R
Ubiquitin ligation to substrate by a cullin-RING E3 ligase & Cdc34: NEDD8-CUL2-RBX1-ELOB/C-FEM1C with trapped UBE2R2~donor UB-Sil1 peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cullin-2 | polymer | 745 | 87098.9 | 1 | UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (human) | CUL-2 |
| 2 | B (H) | Protein fem-1 homolog C | polymer | 617 | 68767.3 | 1 | UniProt (Q96JP0) Pfam (PF12796) | Homo sapiens (human) | FEM1c,FEM1-gamma |
| 3 | C (U) | Ubiquitin | polymer | 685 | 77146.4 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (human) | |
| 4 | D (F) | Nucleotide exchange factor SIL1 | polymer | 20 | 2227.5 | 1 | UniProt (Q9H173) | Homo sapiens (human) | BiP-associated protein,BAP |
| 5 | E (C) | Ubiquitin-conjugating enzyme E2 R2 | polymer | 238 | 27190.9 | 1 | UniProt (Q712K3) Pfam (PF00179) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme R2,Ubiquitin carrier protein R2,Ubiquitin-conjugating enzyme E2-CDC34B,Ubiquitin-protein ligase R2 |
| 6 | F (D) | Elongin-C | polymer | 112 | 12485.1 | 1 | UniProt (Q15369) Pfam (PF03931) | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 7 | G (G) | Elongin-B | polymer | 118 | 13147.8 | 1 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 8 | H (R) | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) Pfam (PF12678) | Homo sapiens (human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 9 | I (U) | 5-azanyl-1-oxidanyl-pentan-2-one | non-polymer | 117.1 | 1 | Chemie (U9O) | |||
| 10 | J, K, L (R) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
F: 442 - 461 (UniProt: Q9H173)
| PDB | External Database | Details |
|---|---|---|
| Cys 442 | Asp 442 | engineered mutation |
| Pro 455 | Ser 455 | engineered mutation |
| Thr 456 | Leu 456 | engineered mutation |
| Gln 457 | Leu 457 | engineered mutation |
| Gly 458 | Lys 458 | engineered mutation |
| Arg 459 | Glu 459 | engineered mutation |
| Ala 460 | Leu 460 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 300354.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 313.4 | |
| All* | Total formula weight | 300667.3 |
