8Q7O

Crystal structure of the FZD3 cysteine-rich domain in complex with a nanobody (14478)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Nanobody 14478	polymer	124	13264.6	2		Lama glama
2	C, D	Frizzled-3	polymer	124	14452.7	2	UniProt (Q9NPG1) Pfam (PF01392) In PDB	Homo sapiens (human)	Fz-3,hFz3
3	A, C	DIMETHYL SULFOXIDE	non-polymer		78.1	2	Chemie (DMS)
4	B, C, D	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	B	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
6	C, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
7	C, D	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
8		water	water		18.0	207	Chemie (HOH)

Sequence modifications

C, D: 26 - 138 (UniProt: Q9NPG1)

PDB	External Database	Details
Glu 23	-	expression tag
Thr 24	-	expression tag
Gly 25	-	expression tag
Gly 139	-	expression tag
Thr 140	-	expression tag
Leu 141	-	expression tag
Glu 142	-	expression tag
Val 143	-	expression tag
Leu 144	-	expression tag
Phe 145	-	expression tag
Gln 146	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	55434.6
Non-Polymers*	Number of molecules	11
	Total formula weight	1355.3
All*	Total formula weight	56789.9

*Water molecules are not included.