8Q7O

Crystal structure of the FZD3 cysteine-rich domain in complex with a nanobody (14478)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	53.659, 147.475, 229.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.170 - 1.760
R-factor	0.2174
Rwork	0.217
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.887
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	1.822
High resolution limit [Å]	1.759	1.759
Number of reflections	90730	8825
<I/σ(I)>	15.4
Completeness [%]	99.5
Redundancy	13.8
CC(1/2)	0.999	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM citric acid, pH 3.6; 690 mM ammonium sulphate