8Q7O
Crystal structure of the FZD3 cysteine-rich domain in complex with a nanobody (14478)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-28 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.659, 147.475, 229.727 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.170 - 1.760 |
R-factor | 0.2174 |
Rwork | 0.217 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.887 |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.170 | 1.822 |
High resolution limit [Å] | 1.759 | 1.759 |
Number of reflections | 90730 | 8825 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 99.5 | |
Redundancy | 13.8 | |
CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100 mM citric acid, pH 3.6; 690 mM ammonium sulphate |