8Q3C

Structure of Selenomonas ruminantium lactate dehydrogenase I85R mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D (H, N, a)	L-lactate dehydrogenase	polymer	318	35061.0	3	UniProt (Q9EVR0) Pfam (PF00056) Pfam (PF02866)	Selenomonas ruminantium
2	C (U)	L-lactate dehydrogenase	polymer	318	35141.0	1	UniProt (Q9EVR0) Pfam (PF00056) Pfam (PF02866)	Selenomonas ruminantium
3	E (H)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	F, H, I (H, N)	NITRATE ION	non-polymer		62.0	3	Chemie (NO3)
5	G (N)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	J, K, L, M (H, N, U, a)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

H, N, a: 1 - 54 (UniProt: Q9EVR0)
H, N, a: 55 - 279 (UniProt: Q9EVR0)

PDB	External Database	Details
Arg 85	Ile 85	engineered mutation

H, N, a: 280 - 318 (UniProt: Q9EVR0)
U: 1 - 54 (UniProt: Q9EVR0)
U: 55 - 279 (UniProt: Q9EVR0)

PDB	External Database	Details
Arg 85	Ile 85	engineered mutation

U: 280 - 318 (UniProt: Q9EVR0)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	140324.0
Non-Polymers*	Number of molecules	5
	Total formula weight	316.4
All*	Total formula weight	140640.5

*Water molecules are not included.