8Q0N

HACE1 in complex with RAC1 Q61L

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	E3 ubiquitin-protein ligase HACE1	polymer	889	99930.7	1	UniProt (Q8IYU2) Pfam (PF12796) Pfam (PF00632)	Homo sapiens (human)	HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1,HECT-type E3 ubiquitin transferase HACE1
2	B (C)	Ras-related C3 botulinum toxin substrate 1	polymer	213	23769.7	1	UniProt (P63000) Pfam (PF00071)	Homo sapiens (human)	Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1
3	C (B)	iodoacetic acid	non-polymer		185.9	1	Chemie (04E)
4	D (C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)

Sequence modifications

B: 22 - 909 (UniProt: Q8IYU2)

PDB	External Database	Details
Gly 21	-	expression tag

C: 1 - 192 (UniProt: P63000)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Leu 61	Gln 61	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	123700.3
Non-Polymers*	Number of molecules	2
	Total formula weight	709.1
All*	Total formula weight	124409.5

*Water molecules are not included.