8POK

Cryo-EM structure of cell-free synthesized human histamine H2 receptor coupled to heterotrimeric Gs protein in lipid environment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Histamine H2 receptor	polymer	375	41997.0	1	UniProt (P25021) Pfam (PF00001) In PDB	Homo sapiens (human)	H2R,HH2R,Gastric receptor I
2	B	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44326.2	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	E	Nanobody35	polymer	138	15140.7	1		Lama glama
6	A	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)

Sequence modifications

A: 7 - 365 (UniProt: P25021)

PDB	External Database	Details
Lys 1	-	expression tag
Pro 2	-	expression tag
Tyr 3	-	expression tag
Asp 4	-	expression tag
Gly 5	-	expression tag
Pro 6	-	expression tag
Gly 366	-	expression tag
Thr 367	-	expression tag
Trp 368	-	expression tag
Ser 369	-	expression tag
His 370	-	expression tag
Pro 371	-	expression tag
Gln 372	-	expression tag
Phe 373	-	expression tag
Glu 374	-	expression tag
Lys 375	-	expression tag

C: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
Gly 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	146755.8
Non-Polymers*	Number of molecules	1
	Total formula weight	111.1
All*	Total formula weight	146867.0

*Water molecules are not included.