8POK
Cryo-EM structure of cell-free synthesized human histamine H2 receptor coupled to heterotrimeric Gs protein in lipid environment
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Histamine H2 receptor | polymer | 375 | 41997.0 | 1 | UniProt (P25021) Pfam (PF00001) In PDB | Homo sapiens (human) | H2R,HH2R,Gastric receptor I |
2 | B | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44326.2 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
4 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
5 | E | Nanobody35 | polymer | 138 | 15140.7 | 1 | Lama glama | ||
6 | A | HISTAMINE | non-polymer | 111.1 | 1 | Chemie (HSM) |
Sequence modifications
A: 7 - 365 (UniProt: P25021)
C: 7 - 345 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Lys 1 | - | expression tag |
Pro 2 | - | expression tag |
Tyr 3 | - | expression tag |
Asp 4 | - | expression tag |
Gly 5 | - | expression tag |
Pro 6 | - | expression tag |
Gly 366 | - | expression tag |
Thr 367 | - | expression tag |
Trp 368 | - | expression tag |
Ser 369 | - | expression tag |
His 370 | - | expression tag |
Pro 371 | - | expression tag |
Gln 372 | - | expression tag |
Phe 373 | - | expression tag |
Glu 374 | - | expression tag |
Lys 375 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 1 | - | expression tag |
Pro 2 | - | expression tag |
Gly 3 | - | expression tag |
Ser 4 | - | expression tag |
Ser 5 | - | expression tag |
Gly 6 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 146755.8 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 111.1 | |
All* | Total formula weight | 146867.0 |