8PM2

Structure of the murine trace amine-associated receptor TAAR7f bound to N,N-dimethylcyclohexylamine (DMCH) in complex with mini-Gs trimeric G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	249	28964.7	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37342.8	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	156	16926.1	1		Lama glama
5	E (R)	Trace amine-associated receptor 7f	polymer	365	41012.5	1	UniProt (Q5QD08) Pfam (PF00001)	Mus musculus (house mouse)	TaR-7f,Trace amine receptor 7f,mTaar7f
6	F (R)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
7	G (R)	~{N},~{N}-dimethylcyclohexanamine	non-polymer		127.2	1	Chemie (8IA)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 1	-	expression tag

G: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

R: 1 - 358 (UniProt: Q5QD08)

PDB	External Database	Details
Ser 0	-	expression tag
Leu 359	-	expression tag
Glu 360	-	expression tag
Val 361	-	expression tag
Leu 362	-	expression tag
Phe 363	-	expression tag
Gln 364	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	132091.2
Non-Polymers*	Number of molecules	2
	Total formula weight	614.0
All*	Total formula weight	132705.2

*Water molecules are not included.