8PKA

Structure of the human mitochondrial iron-sulfur cluster biosynthesis complex during persulfide transfer (without frataxin)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cysteine desulfurase	polymer	404	44937.4	1	UniProt (Q9Y697) Pfam (PF00266) In PDB	Homo sapiens (human)
2	B	LYR motif-containing protein 4	polymer	115	13502.4	1	UniProt (Q9HD34) Pfam (PF05347) In PDB	Homo sapiens (human)
3	C	Acyl carrier protein	polymer	78	8645.5	1	UniProt (P0A6A8) Pfam (PF00550) In PDB	Escherichia coli BL21(DE3)	ACP,Cytosolic-activating factor,CAF,Fatty acid synthase acyl carrier protein
4	D	Iron-sulfur cluster assembly enzyme ISCU	polymer	143	15684.1	1	UniProt (Q9H1K1) Pfam (PF01592) In PDB	Homo sapiens (human)	NifU-like N-terminal domain-containing protein,NifU-like protein
5	A	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	1	Chemie (PLP)
6	C	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	1	Chemie (8Q1)
7	D	FE (II) ION	non-polymer		55.8	1	Chemie (FE2)
8		water	water		18.0	62	Chemie (HOH)

Sequence modifications

A: 56 - 457 (UniProt: Q9Y697)

PDB	External Database	Details
Met 54	-	initiating methionine
Ser 55	-	expression tag

B: 1 - 91 (UniProt: Q9HD34)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag
Asn 0	-	expression tag
Ala 11	Ser 11	conflict

D: 35 - 167 (UniProt: Q9H1K1)

PDB	External Database	Details
Met 33	-	initiating methionine
Ala 34	-	expression tag
Leu 168	-	expression tag
Glu 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	82769.4
Non-Polymers*	Number of molecules	3
	Total formula weight	843.6
All*	Total formula weight	83613.0

*Water molecules are not included.