8PK9
Structure of the human mitochondrial iron-sulfur cluster biosynthesis complex during persulfide transfer (persulfide on NFS1 and ISCU2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cysteine desulfurase | polymer | 404 | 44969.5 | 1 | UniProt (Q9Y697) Pfam (PF00266) | Homo sapiens (human) | |
| 2 | B (B) | LYR motif-containing protein 4 | polymer | 115 | 13502.4 | 1 | UniProt (Q9HD34) Pfam (PF05347) | Homo sapiens (human) | |
| 3 | C (C) | Acyl carrier protein | polymer | 78 | 8645.5 | 1 | UniProt (P0A6A8) Pfam (PF00550) | Escherichia coli BL21(DE3) | ACP,Cytosolic-activating factor,CAF,Fatty acid synthase acyl carrier protein |
| 4 | D (D) | Iron-sulfur cluster assembly enzyme ISCU | polymer | 143 | 15716.2 | 1 | UniProt (Q9H1K1) Pfam (PF01592) | Homo sapiens (human) | NifU-like N-terminal domain-containing protein,NifU-like protein |
| 5 | E (E) | Frataxin mature form | polymer | 133 | 14555.0 | 1 | UniProt (Q16595) Pfam (PF01491) | Homo sapiens (human) | Frataxin(81-210),m81-FXN |
| 6 | F (A) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
| 7 | G (C) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 1 | Chemie (8Q1) | |||
| 8 | H (D) | FE (II) ION | non-polymer | 55.8 | 1 | Chemie (FE2) | |||
| 9 | I, J, K, L (A, B, D, E) | water | water | 18.0 | 99 | Chemie (HOH) |
Sequence modifications
A: 56 - 457 (UniProt: Q9Y697)
B: 1 - 91 (UniProt: Q9HD34)
D: 35 - 167 (UniProt: Q9H1K1)
E: 81 - 210 (UniProt: Q16595)
| PDB | External Database | Details |
|---|---|---|
| Met 54 | - | initiating methionine |
| Ser 55 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | initiating methionine |
| Gly -22 | - | expression tag |
| Ser -21 | - | expression tag |
| Ser -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Pro -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| His -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Ala 11 | Ser 11 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 33 | - | initiating methionine |
| Ala 34 | - | expression tag |
| Leu 168 | - | expression tag |
| Glu 169 | - | expression tag |
| His 170 | - | expression tag |
| His 171 | - | expression tag |
| His 172 | - | expression tag |
| His 173 | - | expression tag |
| His 174 | - | expression tag |
| His 175 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 78 | - | expression tag |
| Asn 79 | - | expression tag |
| Ala 80 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 97388.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 843.6 | |
| All* | Total formula weight | 98232.2 |
