8PI6

Crystal structure of the monomeric zinc free human insulin A22K, B3E, B26E, B29R, desB30 precursor with a Ser-Glu-Asp-Trp-Trp-Arg C-peptide and a Glu-Glu-Gly-Glu-Pro-Arg N-terminal extension

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Insulin B chain,Insulin A chain	polymer	63	7396.3	5	UniProt (P01308) Pfam (PF00049)	Homo sapiens (human)
2	F, G, H, I, J (A, B, C, D, E)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 29 (UniProt: P01308)

PDB	External Database	Details
Glu -5	-	expression tag
Glu -4	-	expression tag
Gly -3	-	expression tag
Glu -2	-	expression tag
Pro -1	-	expression tag
Arg 0	-	expression tag
Glu 3	Asn 27	engineered mutation
Glu 26	Tyr 50	engineered mutation
Arg 29	Lys 53	engineered mutation

A, B, C, D, E: 36 - 56 (UniProt: P01308)

PDB	External Database	Details
Ser 30	-	linker
Glu 31	-	linker
Asp 32	-	linker
Trp 33	-	linker
Trp 34	-	linker
Arg 35	-	linker
Lys 57	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	36981.4
All*	Total formula weight	36981.4

*Water molecules are not included.