8PFG

autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter complex), not fully complementary scaffold

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	P	Transcription antitermination protein RfaH	polymer	164	18531.4	1	UniProt (P0AFW0) Pfam (PF02357) In PDB	Escherichia coli
2	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	J	DNA-directed RNA polymerase subunit beta'	polymer	1416	156505.0	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	G, H	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
6	R	RNA	polymer	17	5382.2	1		Escherichia coli
7	B	template DNA	polymer	40	12446.0	1		Escherichia coli
8	A	non-template DNA	polymer	40	12207.8	1		Escherichia coli
9	J	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
10	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

P: 1 - 162 (UniProt: P0AFW0)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Cys 51	Phe 51	engineered mutation
Cys 139	Ser 139	engineered mutation

J: 2 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Val 1	-	expression tag
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	439260.2
Non-Polymers*	Number of molecules	2
	Total formula weight	89.7
All*	Total formula weight	439349.9

*Water molecules are not included.