8PFG
autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter complex), not fully complementary scaffold
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | Transcription antitermination protein RfaH | polymer | 164 | 18531.4 | 1 | UniProt (P0AFW0) Pfam (PF02357) | Escherichia coli | |
| 2 | B (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | C (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1416 | 156505.0 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | D (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | E, F (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 6 | G (R) | RNA | polymer | 17 | 5382.2 | 1 | Escherichia coli | ||
| 7 | H (B) | template DNA | polymer | 40 | 12446.0 | 1 | Escherichia coli | ||
| 8 | I (A) | non-template DNA | polymer | 40 | 12207.8 | 1 | Escherichia coli | ||
| 9 | J (J) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 10 | K (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
P: 1 - 162 (UniProt: P0AFW0)
J: 2 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Cys 51 | Phe 51 | engineered mutation |
| Cys 139 | Ser 139 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Val 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| His 1414 | - | expression tag |
| His 1415 | - | expression tag |
| His 1416 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 439260.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 89.7 | |
| All* | Total formula weight | 439349.9 |
