8PFG

autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter complex), not fully complementary scaffold

Functional Information from GO Data

Chain	GOid	namespace	contents
G	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006351	biological_process	DNA-templated transcription
G	0006352	biological_process	DNA-templated transcription initiation
G	0006879	biological_process	intracellular iron ion homeostasis
G	0008023	cellular_component	transcription elongation factor complex
G	0009408	biological_process	response to heat
G	0016020	cellular_component	membrane
G	0016779	molecular_function	nucleotidyltransferase activity
G	0031564	biological_process	transcription antitermination
G	0032784	biological_process	regulation of DNA-templated transcription elongation
G	0034062	molecular_function	5'-3' RNA polymerase activity
G	0036460	biological_process	cellular response to cell envelope stress
G	0042128	biological_process	nitrate assimilation
G	0044780	biological_process	bacterial-type flagellum assembly
G	0048870	biological_process	cell motility
G	0071973	biological_process	bacterial-type flagellum-dependent cell motility
G	0090605	biological_process	submerged biofilm formation
G	2000142	biological_process	regulation of DNA-templated transcription initiation
H	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0003677	molecular_function	DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006351	biological_process	DNA-templated transcription
H	0006352	biological_process	DNA-templated transcription initiation
H	0006879	biological_process	intracellular iron ion homeostasis
H	0008023	cellular_component	transcription elongation factor complex
H	0009408	biological_process	response to heat
H	0016020	cellular_component	membrane
H	0016779	molecular_function	nucleotidyltransferase activity
H	0031564	biological_process	transcription antitermination
H	0032784	biological_process	regulation of DNA-templated transcription elongation
H	0034062	molecular_function	5'-3' RNA polymerase activity
H	0036460	biological_process	cellular response to cell envelope stress
H	0042128	biological_process	nitrate assimilation
H	0044780	biological_process	bacterial-type flagellum assembly
H	0048870	biological_process	cell motility
H	0071973	biological_process	bacterial-type flagellum-dependent cell motility
H	0090605	biological_process	submerged biofilm formation
H	2000142	biological_process	regulation of DNA-templated transcription initiation
I	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
I	0000428	cellular_component	DNA-directed RNA polymerase complex
I	0003677	molecular_function	DNA binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006351	biological_process	DNA-templated transcription
I	0006352	biological_process	DNA-templated transcription initiation
I	0006879	biological_process	intracellular iron ion homeostasis
I	0008023	cellular_component	transcription elongation factor complex
I	0009408	biological_process	response to heat
I	0016020	cellular_component	membrane
I	0016779	molecular_function	nucleotidyltransferase activity
I	0031564	biological_process	transcription antitermination
I	0032784	biological_process	regulation of DNA-templated transcription elongation
I	0034062	molecular_function	5'-3' RNA polymerase activity
I	0036460	biological_process	cellular response to cell envelope stress
I	0042128	biological_process	nitrate assimilation
I	0044780	biological_process	bacterial-type flagellum assembly
I	0046677	biological_process	response to antibiotic
I	0048870	biological_process	cell motility
I	0071973	biological_process	bacterial-type flagellum-dependent cell motility
I	0090605	biological_process	submerged biofilm formation
I	2000142	biological_process	regulation of DNA-templated transcription initiation
J	0000287	molecular_function	magnesium ion binding
J	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006351	biological_process	DNA-templated transcription
J	0006352	biological_process	DNA-templated transcription initiation
J	0006879	biological_process	intracellular iron ion homeostasis
J	0008023	cellular_component	transcription elongation factor complex
J	0008270	molecular_function	zinc ion binding
J	0009408	biological_process	response to heat
J	0016020	cellular_component	membrane
J	0016779	molecular_function	nucleotidyltransferase activity
J	0031564	biological_process	transcription antitermination
J	0032784	biological_process	regulation of DNA-templated transcription elongation
J	0034062	molecular_function	5'-3' RNA polymerase activity
J	0036460	biological_process	cellular response to cell envelope stress
J	0042128	biological_process	nitrate assimilation
J	0044780	biological_process	bacterial-type flagellum assembly
J	0046677	biological_process	response to antibiotic
J	0046872	molecular_function	metal ion binding
J	0048870	biological_process	cell motility
J	0071973	biological_process	bacterial-type flagellum-dependent cell motility
J	0090605	biological_process	submerged biofilm formation
J	2000142	biological_process	regulation of DNA-templated transcription initiation
K	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005829	cellular_component	cytosol
K	0006351	biological_process	DNA-templated transcription
K	0006352	biological_process	DNA-templated transcription initiation
K	0006879	biological_process	intracellular iron ion homeostasis
K	0008023	cellular_component	transcription elongation factor complex
K	0009408	biological_process	response to heat
K	0016779	molecular_function	nucleotidyltransferase activity
K	0030880	cellular_component	RNA polymerase complex
K	0031564	biological_process	transcription antitermination
K	0032784	biological_process	regulation of DNA-templated transcription elongation
K	0034062	molecular_function	5'-3' RNA polymerase activity
K	0036460	biological_process	cellular response to cell envelope stress
K	0042128	biological_process	nitrate assimilation
K	0044780	biological_process	bacterial-type flagellum assembly
K	0048870	biological_process	cell motility
K	0065003	biological_process	protein-containing complex assembly
K	0071973	biological_process	bacterial-type flagellum-dependent cell motility
K	0090605	biological_process	submerged biofilm formation
K	2000142	biological_process	regulation of DNA-templated transcription initiation
P	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
P	0001073	molecular_function	transcription antitermination factor activity, DNA binding
P	0003677	molecular_function	DNA binding
P	0005515	molecular_function	protein binding
P	0005829	cellular_component	cytosol
P	0006354	biological_process	DNA-templated transcription elongation
P	0008494	molecular_function	translation activator activity
P	0031564	biological_process	transcription antitermination
P	0045727	biological_process	positive regulation of translation
P	0061980	molecular_function	regulatory RNA binding

Functional Information from PROSITE/UniProt

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV

Chain	Residue	Details
I	GLY1063-VAL1075

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: ADP-ribosylarginine => ECO:0000269|PubMed:4371081

Chain	Residue	Details
G	ARG265
J	CYS895
J	CYS898
H	ARG265
J	CYS85
J	CYS88
J	ASP460
J	ASP462
J	ASP464
J	CYS814
J	CYS888

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site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:21696463

Chain	Residue	Details
G	LYS297
H	LYS297

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site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine; by PatZ => ECO:0000269|PubMed:21696463

Chain	Residue	Details
G	LYS298
H	LYS298

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