8P9U

Crystal Structure of Two-Domain Laccase mutant M199A/D268N from Streptomyces griseoflavus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E, F	Two-domain laccase	polymer	278	30227.6	5	UniProt (A0A0M4FJ81) Pfam (PF07732) Pfam (PF07731) In PDB	Streptomyces griseoflavus
2	C	Two-domain laccase	polymer	278	30284.7	1	UniProt (A0A0M4FJ81) Pfam (PF07732) Pfam (PF07731) In PDB	Streptomyces griseoflavus
3	E, F, A, B, C...	COPPER (II) ION	non-polymer		63.5	20	Chemie (CU)
4	F, A, B, C, D	OXYGEN MOLECULE	non-polymer		32.0	5	Chemie (OXY)
5	F, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6		water	water		18.0	758	Chemie (HOH)

Sequence modifications

A, B, D, E, F: 40 - 317 (UniProt: A0A0M4FJ81)

PDB	External Database	Details
Ala 199	Met 199	engineered mutation
Asn 268	Asp 268	engineered mutation

C: 41 - 318 (UniProt: A0A0M4FJ81)

PDB	External Database	Details
Ala 199	Met 199	engineered mutation
Asn 268	Asp 268	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	181422.7
Non-Polymers*	Number of molecules	27
	Total formula weight	1511.1
All*	Total formula weight	182933.8

*Water molecules are not included.