8P9U
Crystal Structure of Two-Domain Laccase mutant M199A/D268N from Streptomyces griseoflavus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, D, E, F (A, B, D, E, F) | Two-domain laccase | polymer | 278 | 30227.6 | 5 | UniProt (A0A0M4FJ81) Pfam (PF07732) Pfam (PF07731) | Streptomyces griseoflavus | |
| 2 | C (C) | Two-domain laccase | polymer | 278 | 30284.7 | 1 | UniProt (A0A0M4FJ81) Pfam (PF07732) Pfam (PF07731) | Streptomyces griseoflavus | |
| 3 | AA, BA, CA, DA, EA... (E, F, A, B, C...) | COPPER (II) ION | non-polymer | 63.5 | 20 | Chemie (CU) | |||
| 4 | FA, J, N, T, X (F, A, B, C, D) | OXYGEN MOLECULE | non-polymer | 32.0 | 5 | Chemie (OXY) | |||
| 5 | GA, O (F, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | HA, IA, JA, KA, LA... (A, B, C, D, E...) | water | water | 18.0 | 758 | Chemie (HOH) |
Sequence modifications
A, B, D, E, F: 40 - 317 (UniProt: A0A0M4FJ81)
C: 41 - 318 (UniProt: A0A0M4FJ81)
| PDB | External Database | Details |
|---|---|---|
| Ala 199 | Met 199 | engineered mutation |
| Asn 268 | Asp 268 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 199 | Met 199 | engineered mutation |
| Asn 268 | Asp 268 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 181422.7 | |
| Non-Polymers* | Number of molecules | 27 |
| Total formula weight | 1511.1 | |
| All* | Total formula weight | 182933.8 |
