8P9U
Crystal Structure of Two-Domain Laccase mutant M199A/D268N from Streptomyces griseoflavus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU PhotonJet-S |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2021-03-18 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.302, 94.026, 118.927 |
Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
Resolution | 23.540 - 2.000 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.895 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | MOLREP (11.6.03) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.540 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 104468 | 10454 |
<I/σ(I)> | 8.01 | 1.43 |
Completeness [%] | 94.6 | 95.3 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.980 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 296 | 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate condition #31 of BCS-1 from Molecular Dimensions, UK |