8P5R

Crystal structure of full-length, homohexameric 2-oxoglutarate dehydrogenase KGD from Mycobacterium smegmatis in complex with GarA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Multifunctional 2-oxoglutarate metabolism enzyme	polymer	1250	138548.1	10	UniProt (A0R2B1) Pfam (PF16078) Pfam (PF00198) Pfam (PF00676) Pfam (PF02779) Pfam (PF16870) In PDB	Mycolicibacterium smegmatis MC2 155	2-hydroxy-3-oxoadipate synthase,HOA synthase,HOAS,2-oxoglutarate carboxy-lyase,2-oxoglutarate decarboxylase,Alpha-ketoglutarate decarboxylase,KG decarboxylase,KGD,Alpha-ketoglutarate-glyoxylate carboligase
2	G, H, I, J, K...	Glycogen accumulation regulator GarA	polymer	158	16599.0	6	UniProt (A0QYG2) Pfam (PF00498) In PDB	Mycolicibacterium smegmatis MC2 155
3	D, E, F, A, B...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
4	D, E, F, A, B...	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
5	C, D, E, F, A...	THIAMINE DIPHOSPHATE	non-polymer		425.3	6	Chemie (TPP)

Sequence modifications

A, B, C, D, E, F, N, O, P, Q: 2 - 1227 (UniProt: A0R2B1)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	1485075.0
Non-Polymers*	Number of molecules	20
	Total formula weight	3018.3
All*	Total formula weight	1488093.4

*Water molecules are not included.