8P53

Cryo-EM structure of the c-di-GMP-free FleQ-FleN master regulator complex of P. aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Antiactivator FleN	polymer	307	33236.3	2	UniProt (G3XD64) Pfam (PF10609)	Pseudomonas aeruginosa PAO1
2	C, D, E, F (C, D, E, F)	Transcriptional regulator FleQ	polymer	492	55494.1	4	UniProt (G3XCV0) Pfam (PF06490) Pfam (PF00158) Pfam (PF02954)	Pseudomonas aeruginosa PAO1
3	G, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	H, J (A, B)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	2	Chemie (ACP)

Sequence modifications

A, B: 2 - 280 (UniProt: G3XD64)

PDB	External Database	Details
Met -26	-	initiating methionine
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 48	Asp 48	engineered mutation

C, D, E, F: 2 - 490 (UniProt: G3XCV0)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	288449.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1059.0
All*	Total formula weight	289508.2

*Water molecules are not included.