8P12

Cryo-EM structure of Rhodopsin-Gi bound to antibody fragment Fab13

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Rhodopsin	polymer	348	39040.5	1	UniProt (P02699) Pfam (PF10413) Pfam (PF00001)	Bos taurus (cattle)
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	376	43182.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (cattle)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(T) subunit gamma-T1	polymer	74	8556.9	1	UniProt (P02698) Pfam (PF00631)	Bos taurus (cattle)	Transducin gamma chain
5	E (L)	Immunoglobin G light chain	polymer	238	26349.6	1		Mus musculus
6	F (H)	Immunoglobin G heavy chain FAB fragment	polymer	258	27448.3	1		Mus musculus

Sequence modifications

R: 1 - 348 (UniProt: P02699)

PDB	External Database	Details
Cys 2	Asn 2	engineered mutation
Tyr 257	Met 257	engineered mutation
Cys 282	Asp 282	engineered mutation

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Met -21	-	initiating methionine
Lys -20	-	expression tag
Lys -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	181994.3
All*	Total formula weight	181994.3

*Water molecules are not included.