8OYP

Crystal structure of Ubiquitin specific protease 11 (USP11) in complex with a substrate mimetic

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ubiquitin carboxyl-terminal hydrolase 11,Response regulator FrzS	polymer	485	55166.9	2	UniProt (P51784) UniProt (Q1D4U9) Pfam (PF00443) Pfam (PF00072)	Homo sapiens (human)	Deubiquitinating enzyme 11,Ubiquitin thioesterase 11,Ubiquitin-specific-processing protease 11
2	C, D (C, D)	Polyubiquitin-B	polymer	79	8748.0	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (human)
3	E, J (A, B)	CADMIUM ION	non-polymer		112.4	2	Chemie (CD)
4	F, K (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	G, H, L, M (A, B)	NITRATE ION	non-polymer		62.0	4	Chemie (NO3)
6	I, P (A, D)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	N, O (B, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	Q, R, S, T (A, B, C, D)	water	water		18.0	505	Chemie (HOH)

Sequence modifications

A, B: 295 - 489 (UniProt: P51784)

PDB	External Database	Details
Ser 318	Cys 318	engineered mutation

A, B: 492 - 606 (UniProt: Q1D4U9)

PDB	External Database	Details
Ala 583	Lys 94	conflict
Ser 585	Lys 96	conflict
Ala 607	-	linker
Leu 608	-	linker
Ile 609	-	linker
Gly 610	-	linker
Phe 611	-	linker
Pro 612	-	linker

A, B: 778 - 937 (UniProt: P51784)

PDB	External Database	Details
Gly 490	-	linker
Ala 491	-	linker
Glu 938	-	expression tag
His 939	-	expression tag
His 940	-	expression tag
His 941	-	expression tag
His 942	-	expression tag
His 943	-	expression tag
His 944	-	expression tag

C, D: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly 77	-	expression tag
Gly 78	-	expression tag
Gly 79	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127829.9
Non-Polymers*	Number of molecules	12
	Total formula weight	917.9
All*	Total formula weight	128747.7

*Water molecules are not included.