8OXS

Cholera holotoxin variant (chimera with E. coli heat-labile enterotoxin, 4 C-terminal substitutions)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G (A, B)	Cholera enterotoxin subunit A	polymer	240	27266.1	2	UniProt (P01555) Pfam (PF01375)	Vibrio cholerae O1	Cholera enterotoxin,A chain
2	B, C, D, E, F... (D, E, F, G, H...)	Cholera enterotoxin subunit B	polymer	103	11623.3	10	UniProt (P01556) Pfam (PF01376)	Vibrio cholerae O1	Cholera enterotoxin B chain,Cholera enterotoxin gamma chain,Choleragenoid
3	M, Z (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	CA, EA, GA, IA, N... (C, I, K, L, A...)	beta-D-galactopyranose	non-polymer		180.2	10	Chemie (GAL)
5	BA, FA, HA, O, R... (C, K, L, D, E...)	alpha-D-galactopyranose	non-polymer		180.2	7	Chemie (GLA)
6	AA, DA, Q, W (B, I, E, H)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	4	Chemie (EPE)
7	JA, KA, LA, MA, NA... (A, D, E, F, G...)	water	water		18.0	764	Chemie (HOH)

Sequence modifications

A, B: 1 - 240 (UniProt: P01555)

PDB	External Database	Details
Glu 229	Asp 247	engineered mutation
Val 230	Ile 248	engineered mutation
Ile 232	Thr 250	engineered mutation
Tyr 233	His 251	engineered mutation

D, E, F, G, H, C, I, J, K, L: 1 - 103 (UniProt: P01556)

PDB	External Database	Details
His 18	Tyr 39	engineered mutation
Thr 47	Ile 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	170764.8
Non-Polymers*	Number of molecules	23
	Total formula weight	4061.9
All*	Total formula weight	174826.6

*Water molecules are not included.