8OVY

Structure of analogue of superfolded GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	247	27983.3	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	C, D (A, B)	water	water		18.0	159	Chemie (HOH)

Sequence modifications

A, B: 2 - 304 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 65	Ser 65	chromophore
Cro 65	Tyr 66	chromophore
Cro 65	Gly 67	chromophore
Arg 146	Gln 80	engineered mutation
Ser 165	Phe 99	engineered mutation
Thr 171	Asn 105	engineered mutation
Phe 211	Tyr 145	engineered mutation
W4t 215	Asn 149	engineered mutation
Thr 219	Met 153	engineered mutation
Ala 229	Val 163	engineered mutation
Val 237	Ile 171	engineered mutation
Val 272	Ala 206	engineered mutation
Gly 305	-	expression tag
Ser 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55966.6
All*	Total formula weight	55966.6

*Water molecules are not included.