8OVY

Structure of analogue of superfolded GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-22
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.84
Spacegroup name	C 1 2 1
Unit cell lengths	130.516, 37.721, 91.795
Unit cell angles	90.00, 106.38, 90.00

Refinement procedure

Resolution	88.071 - 1.537
Rwork	0.186
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.789
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.071	1.595
High resolution limit [Å]	1.537	1.537
Rmeas	0.110	1.350
Number of reflections	59575	5803
<I/σ(I)>	5.1
Completeness [%]	93.1
Redundancy	3.3
CC(1/2)	0.990	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350/PEG 1K/MPD (1:1:1) 37.5%, Bicine/Trizma 0.1 M pH 8.5, Morpheus III Alkaloids 0.8% w/v, Morpheus Alcohols 0.12 M