8OTR

SARS-CoV-2 nsp10-16 methyltransferase in complex with SAM analog BDH 33959089

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyltransferase nsp16	polymer	304	34150.1	1	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2	Non-structural protein 16,nsp16
2	B (B)	Non-structural protein 10	polymer	140	14860.0	1	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2	nsp10,Growth factor-like peptide,GFL
3	AA, BA, C, CA, E... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	28	Chemie (EDO)
4	D (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
5	O (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
6	DA (A)	(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-N-(1-methylpiperidin-4-yl)-3,4-bis(oxidanyl)oxolane-2-carboxamide	non-polymer		377.4	1	Chemie (W08)
7	FA (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	GA, HA (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	KA, LA (A, B)	water	water		18.0	262	Chemie (HOH)

Sequence modifications

A: 6799 - 7096 (UniProt: P0DTD1)

PDB	External Database	Details
Glu 7097	-	expression tag
Asn 7098	-	expression tag
Leu 7099	-	expression tag
Tyr 7100	-	expression tag
Phe 7101	-	expression tag
Gln 7102	-	expression tag

B: 4254 - 4392 (UniProt: P0DTD1)

PDB	External Database	Details
Gly 4253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49010.1
Non-Polymers*	Number of molecules	34
	Total formula weight	2875.2
All*	Total formula weight	51885.3

*Water molecules are not included.