8KH5

Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable G-protein coupled receptor 174	polymer	340	39454.3	1	UniProt (Q9BXC1) Pfam (PF00001)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	28907.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39280.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
4	D (E)	Nanobody 35	polymer	160	17352.5	1		Lama glama
5	E (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F, H (A)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
7	G (A)	O-{HYDROXY[((2R)-2-HYDROXY-3-{[(1S)-1-HYDROXYPENTADECYL]OXY}PROPYL)OXY]PHOSPHORYL}-L-SERINE	non-polymer		485.5	1	Chemie (LPS)

Sequence modifications

A: 2 - 328 (UniProt: Q9BXC1)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ala 1	-	expression tag
Glu 324	Thr 324	conflict
Leu 325	Ala 325	conflict
Glu 326	Ser 326	conflict
Val 327	Thr 327	conflict
Leu 328	Met 328	conflict
Phe 329	-	expression tag
Gln 330	-	expression tag
Gly 331	-	expression tag
Pro 332	-	expression tag

B: 6 - 195 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

B: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Arg 145	Tyr 145	engineered mutation

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	132840.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1258.9
All*	Total formula weight	134099.4

*Water molecules are not included.