8KH5
Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Probable G-protein coupled receptor 174 | polymer | 340 | 39454.3 | 1 | UniProt (Q9BXC1) Pfam (PF00001) In PDB | Homo sapiens (human) | |
2 | B | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 248 | 28907.7 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 357 | 39280.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | |
4 | E | Nanobody 35 | polymer | 160 | 17352.5 | 1 | Lama glama | ||
5 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
6 | A | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
7 | A | O-{HYDROXY[((2R)-2-HYDROXY-3-{[(1S)-1-HYDROXYPENTADECYL]OXY}PROPYL)OXY]PHOSPHORYL}-L-SERINE | non-polymer | 485.5 | 1 | Chemie (LPS) |
Sequence modifications
A: 2 - 328 (UniProt: Q9BXC1)
B: 6 - 195 (UniProt: P63092)
B: 204 - 394 (UniProt: P63092)
C: 2 - 340 (UniProt: P62873)
D: 1 - 71 (UniProt: P59768)
PDB | External Database | Details |
---|---|---|
Asp -7 | - | expression tag |
Tyr -6 | - | expression tag |
Lys -5 | - | expression tag |
Asp -4 | - | expression tag |
Asp -3 | - | expression tag |
Asp -2 | - | expression tag |
Asp -1 | - | expression tag |
Lys 0 | - | expression tag |
Ala 1 | - | expression tag |
Glu 324 | Thr 324 | conflict |
Leu 325 | Ala 325 | conflict |
Glu 326 | Ser 326 | conflict |
Val 327 | Thr 327 | conflict |
Leu 328 | Met 328 | conflict |
Phe 329 | - | expression tag |
Gln 330 | - | expression tag |
Gly 331 | - | expression tag |
Pro 332 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Asp 49 | Gly 49 | engineered mutation |
Asn 50 | Glu 50 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 196 | - | linker |
Gly 197 | - | linker |
Ser 198 | - | linker |
Gly 199 | - | linker |
Gly 200 | - | linker |
Ser 201 | - | linker |
Gly 202 | - | linker |
Gly 203 | - | linker |
Asp 249 | Ala 249 | engineered mutation |
Asp 252 | Ser 252 | engineered mutation |
- | Asn 254 | deletion |
- | Met 255 | deletion |
- | Val 256 | deletion |
- | Ile 257 | deletion |
- | Arg 258 | deletion |
- | Glu 259 | deletion |
- | Asp 260 | deletion |
- | Asn 261 | deletion |
- | Gln 262 | deletion |
- | Thr 263 | deletion |
Ala 372 | Ile 372 | conflict |
Ile 375 | Val 375 | conflict |
PDB | External Database | Details |
---|---|---|
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
Arg 145 | Tyr 145 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 132840.5 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 1258.9 | |
All* | Total formula weight | 134099.4 |