8KH4
Cryo-EM structure of the GPR161-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | G-protein coupled receptor 161 | polymer | 373 | 41999.1 | 1 | UniProt (Q8N6U8) Pfam (PF00001) In PDB | Homo sapiens (human) | |
2 | B | Guanine nucleotide-binding protein G(olf) subunit alpha,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 249 | 29008.8 | 1 | UniProt (P38405) UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein,olfactory type,Adenylate cyclase-stimulating G alpha protein |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 357 | 39280.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | |
4 | E | Nanobody 35 | polymer | 160 | 17352.5 | 1 | Lama glama | ||
5 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
6 | A | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
A: 2 - 348 (UniProt: Q8N6U8)
B: 5 - 195 (UniProt: P38405)
B: 204 - 394 (UniProt: P63092)
C: 2 - 340 (UniProt: P62873)
D: 1 - 71 (UniProt: P59768)
PDB | External Database | Details |
---|---|---|
Asp -7 | - | expression tag |
Tyr -6 | - | expression tag |
Lys -5 | - | expression tag |
Asp -4 | - | expression tag |
Asp -3 | - | expression tag |
Asp -2 | - | expression tag |
Asp -1 | - | expression tag |
Lys 0 | - | expression tag |
Ala 1 | - | expression tag |
Glu 349 | - | expression tag |
Leu 350 | - | expression tag |
Glu 351 | - | expression tag |
Val 352 | - | expression tag |
Leu 353 | - | expression tag |
Phe 354 | - | expression tag |
Gln 355 | - | expression tag |
Gly 356 | - | expression tag |
Pro 357 | - | expression tag |
Leu 358 | - | expression tag |
Glu 359 | - | expression tag |
Val 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Gln 363 | - | expression tag |
Gly 364 | - | expression tag |
Pro 365 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Arg 13 | Gly 15 | engineered mutation |
Asn 14 | Val 16 | engineered mutation |
Glu 15 | Asp 17 | engineered mutation |
Ala 18 | Glu 20 | engineered mutation |
Gln 19 | Arg 21 | engineered mutation |
Asp 33 | Glu 35 | engineered mutation |
Lys 34 | Arg 36 | engineered mutation |
Gln 35 | Leu 37 | engineered mutation |
Val 36 | Ala 38 | engineered mutation |
Arg 38 | Lys 40 | engineered mutation |
Asp 49 | Gly 51 | engineered mutation |
Asn 50 | Glu 52 | engineered mutation |
Gly 196 | - | linker |
Gly 197 | - | linker |
Ser 198 | - | linker |
Gly 199 | - | linker |
Gly 200 | - | linker |
Ser 201 | - | linker |
Gly 202 | - | linker |
Gly 203 | - | linker |
PDB | External Database | Details |
---|---|---|
Asp 249 | Ala 249 | engineered mutation |
Asp 252 | Ser 252 | engineered mutation |
- | Asn 254 | deletion |
- | Met 255 | deletion |
- | Val 256 | deletion |
- | Ile 257 | deletion |
- | Arg 258 | deletion |
- | Glu 259 | deletion |
- | Asp 260 | deletion |
- | Asn 261 | deletion |
- | Gln 262 | deletion |
- | Thr 263 | deletion |
Ala 372 | Ile 372 | engineered mutation |
Ile 375 | Val 375 | engineered mutation |
PDB | External Database | Details |
---|---|---|
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
Arg 145 | Tyr 145 | conflict |
PDB | External Database | Details |
---|---|---|
Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 135486.4 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 386.7 | |
All* | Total formula weight | 135873.0 |