8K9K

Full agonist-bound mu-type opioid receptor-G protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Soluble cytochrome b562,Mu-type opioid receptor	polymer	453	51321.9	1	UniProt (P0ABE7) UniProt (P35372) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP
2	S	DAMGO	polymer	5	513.6	1	BIRD (PRD_002308)	synthetic construct
3	A	G protein subunit alpha i3	polymer	354	40585.1	1	UniProt (F6VL43) Pfam (PF00503) In PDB	Macaca mulatta (Rhesus monkey)	Guanine nucleotide-binding protein G(K) subunit alpha
4	B	Guanine nucleotide binding protein, beta polypeptide 1	polymer	348	38522.1	1	UniProt (M1ERZ7) Pfam (PF00400) UniProt (by SIFTS) (P62873) In PDB	Homo sapiens (human)
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
6	H	scFv16	polymer	257	27409.6	1		synthetic construct

Sequence modifications

R: -52 - 53 (UniProt: P0ABE7)

PDB	External Database	Details
Cys -74	-	expression tag
Leu -73	-	expression tag
Val -72	-	expression tag
Phe -71	-	expression tag
Ala -70	-	expression tag
Asp -69	-	expression tag
Tyr -68	-	expression tag
Lys -67	-	expression tag
Asp -66	-	expression tag
Asp -65	-	expression tag
Asp -64	-	expression tag
Asp -63	-	expression tag
Ala -62	-	expression tag
Leu -61	-	expression tag
Glu -60	-	expression tag
Val -59	-	expression tag
Leu -58	-	expression tag
Phe -57	-	expression tag
Gln -56	-	expression tag
Gly -55	-	expression tag
Pro -54	-	expression tag
Met -53	-	expression tag
Trp -46	Met 29	engineered mutation
Ile 49	His 124	engineered mutation
Leu 53	Arg 128	engineered mutation
Gly 54	-	linker
Gly 55	-	linker
Ser 56	-	linker
Pro 57	-	linker
Gly 58	-	linker
Ala 59	-	linker
Arg 60	-	linker
Ser 61	-	linker
Ala 62	-	linker
Ser 63	-	linker

R: 64 - 362 (UniProt: P35372)

PDB	External Database	Details
Trp 158	Phe 158	conflict
Leu 363	-	expression tag
Glu 364	-	expression tag
Val 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Gln 368	-	expression tag
Gly 369	-	expression tag
Pro 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

B: 2 - 339 (UniProt: M1ERZ7)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Asn 340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	166213.4
All*	Total formula weight	166213.4

*Water molecules are not included.