8K60
Cryo-EM structure of Streptomyces coelicolor transcription initiation complex with the global transcription factor AfsR
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, K (A, B, K) | DNA-directed RNA polymerase subunit alpha | polymer | 340 | 36734.6 | 3 | UniProt (P60312) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1161 | 128644.9 | 1 | UniProt (Q9L0L0) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1299 | 144807.1 | 1 | UniProt (Q8CJT1) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 9716.9 | 1 | UniProt (Q9KXS1) Pfam (PF01192) | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase principal sigma factor HrdB | polymer | 511 | 56017.6 | 1 | UniProt (P18183) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | RNA polymerase sigma factor SigA |
| 6 | G (G) | Non-template strand DNA for AfsS promoter | polymer | 59 | 18177.6 | 1 | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | ||
| 7 | H (H) | Template strand DNA for AfsS promoter | polymer | 59 | 18360.8 | 1 | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | ||
| 8 | I, J (I, J) | Regulatory protein AfsR | polymer | 993 | 105842.6 | 2 | UniProt (P25941) Pfam (PF00486) Pfam (PF03704) Pfam (PF00931) Pfam (PF13424) Pfam (PF13181) | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) | |
| 9 | L (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | M, N (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 697614.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 697769.1 |
