8JZZ

Structure of human C5a-desArg bound human C5aR1 in complex with Go

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
2	C	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	240	27024.8	1	UniProt (P09471) Pfam (PF00503) In PDB	Homo sapiens (human)
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	H	Antibody fragment ScFv16	polymer	248	26466.5	1		Mus musculus (house mouse)
5	A	C5a anaphylatoxin chemotactic receptor 1	polymer	406	45273.9	1	UniProt (P21730) Pfam (PF00001) In PDB	Homo sapiens (human)	C5a anaphylatoxin chemotactic receptor,C5a-R,C5aR
6	D	C5a anaphylatoxin	polymer	74	8288.7	1	UniProt (P01031) Pfam (PF01821) In PDB	Homo sapiens (human)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

C: 4 - 173 (UniProt: P09471)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Gly 3	-	expression tag
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation

C: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation
-	Asp 232	deletion
-	Gln 233	deletion
-	Val 234	deletion
-	Leu 235	deletion
-	His 236	deletion
-	Glu 237	deletion
-	Asp 238	deletion
-	Glu 239	deletion
-	Thr 240	deletion
-	Thr 241	deletion
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

A: 2 - 350 (UniProt: P21730)

PDB	External Database	Details
Met -55	-	initiating methionine
Gly -54	-	expression tag
Lys -53	-	expression tag
Thr -52	-	expression tag
Ile -51	-	expression tag
Ile -50	-	expression tag
Ala -49	-	expression tag
Leu -48	-	expression tag
Ser -47	-	expression tag
Tyr -46	-	expression tag
Ile -45	-	expression tag
Phe -44	-	expression tag
Cys -43	-	expression tag
Leu -42	-	expression tag
Val -41	-	expression tag
Phe -40	-	expression tag
Ala -39	-	expression tag
Asp -38	-	expression tag
Tyr -37	-	expression tag
Lys -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Ala -31	-	expression tag
Ala -30	-	expression tag
Asn -29	-	expression tag
Phe -28	-	expression tag
Thr -27	-	expression tag
Pro -26	-	expression tag
Val -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Asn -19	-	expression tag
Gln -18	-	expression tag
Ser -17	-	expression tag
Val -16	-	expression tag
Arg -15	-	expression tag
Leu -14	-	expression tag
Val -13	-	expression tag
Thr -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	153449.0
All*	Total formula weight	153449.0

*Water molecules are not included.