8JZ7
Cryo-EM structure of MK-6892-bound HCAR2 in complex with Gi protein
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
2 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
3 | D | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
4 | S | scFv16 | polymer | 266 | 28636.8 | 1 | Mus musculus | ||
5 | A | Hydroxycarboxylic acid receptor 2 | polymer | 397 | 46034.1 | 1 | UniProt (Q8TDS4) Pfam (PF00001) In PDB | Homo sapiens (human) | G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor |
6 | A | 2-[[2,2-dimethyl-3-[3-(5-oxidanylpyridin-2-yl)-1,2,4-oxadiazol-5-yl]propanoyl]amino]cyclohexene-1-carboxylic acid | non-polymer | 386.4 | 1 | Chemie (FI7) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
D: 1 - 354 (UniProt: P63096)
A: 1 - 363 (UniProt: Q8TDS4)
PDB | External Database | Details |
---|---|---|
Met -15 | - | initiating methionine |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Asn 47 | Ser 47 | engineered mutation |
Ala 203 | Gly 203 | engineered mutation |
Ala 245 | Glu 245 | engineered mutation |
Ser 326 | Ala 326 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -33 | - | initiating methionine |
Lys -32 | - | expression tag |
Thr -31 | - | expression tag |
Ile -30 | - | expression tag |
Ile -29 | - | expression tag |
Ala -28 | - | expression tag |
Leu -27 | - | expression tag |
Ser -26 | - | expression tag |
Tyr -25 | - | expression tag |
Ile -24 | - | expression tag |
Phe -23 | - | expression tag |
Cys -22 | - | expression tag |
Leu -21 | - | expression tag |
Val -20 | - | expression tag |
Phe -19 | - | expression tag |
Ala -18 | - | expression tag |
Asp -17 | - | expression tag |
Tyr -16 | - | expression tag |
Lys -15 | - | expression tag |
Asp -14 | - | expression tag |
Asp -13 | - | expression tag |
Asp -12 | - | expression tag |
Asp -11 | - | expression tag |
Ala -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 162087.8 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 386.4 | |
All* | Total formula weight | 162474.2 |