8JYX

Crystal structure of the gasdermin-like protein RCD-1-1 from Neurospora crassa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Maltodextrin-binding protein,Gasdermin-like protein rcd-1-1	polymer	635	69615.4	2	UniProt (A0A376KDN7) UniProt (Q7SBA0) In PDB	Escherichia coli	Regulator of cell death 1-1
2	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
3		water	water		18.0	162	Chemie (HOH)

Sequence modifications

A, B: -372 - -7 (UniProt: A0A376KDN7)

PDB	External Database	Details
Ser -377	-	expression tag
Gly -376	-	expression tag
Arg -375	-	expression tag
Pro -374	-	expression tag
Met -373	-	expression tag
Ala -291	Asp 108	engineered mutation
Ala -290	Lys 109	engineered mutation
Ala -201	Glu 198	engineered mutation
Ala -200	Asn 199	engineered mutation
Ala -134	Lys 265	engineered mutation
Ala -11	Lys 388	engineered mutation
Ala -10	Asp 389	engineered mutation
Asn -6	-	linker
Ala -5	-	linker
Ala -4	-	linker
Arg -3	-	linker
Ala -2	-	linker
Ala -1	-	linker
Ala 0	-	linker

A, B: 1 - 257 (UniProt: Q7SBA0)

PDB	External Database	Details
Ala 174	Lys 174	engineered mutation
Ala 175	Lys 175	engineered mutation
Ala 176	Lys 176	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	139230.7
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	139915.3

*Water molecules are not included.