8JYX

Crystal structure of the gasdermin-like protein RCD-1-1 from Neurospora crassa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	81.144, 91.911, 105.868
Unit cell angles	90.00, 101.38, 90.00

Refinement procedure

Resolution	45.960 - 2.350
R-factor	0.2175
Rwork	0.216
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.550	2.410
High resolution limit [Å]	2.350	2.350
Rmerge	0.090	0.820
Rmeas	0.098	0.886
Rpim	0.037	0.465
Number of reflections	63588	4459
<I/σ(I)>	12.3	2.2
Completeness [%]	99.9	100
Redundancy	6.8	6.9
CC(1/2)	0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	0.1 M Bicine pH 7.4, 11% PEG 3350, 3% (w/v) D(+)-Glucose monohydrate