8JX2

alpha-Hemolysin(G122S/K147R)-SpyTag/SpyCatcher head to head 14-mer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	alpha hemolysin fused with spy-catcher	polymer	421	47003.5	7	UniProt (P09616) Pfam (PF07968)	Staphylococcus aureus	Alpha-HL,Alpha-toxin
2	H, I, J, K, L... (H, I, J, K, L...)	alpha hemolysin fused with spy-tag	polymer	324	36803.9	7	UniProt (P09616) Pfam (PF07968)	Staphylococcus aureus	Alpha-HL,Alpha-toxin

Sequence modifications

A, B, C, D, E, F, G: 1 - 293 (UniProt: P09616)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 122	Gly 148	engineered mutation
Arg 147	Lys 173	engineered mutation
Gly 294	-	expression tag
Ser 295	-	expression tag
Ser 296	-	expression tag
Gly 297	-	expression tag
Ser 298	-	expression tag
Val 299	-	expression tag
Thr 300	-	expression tag
Thr 301	-	expression tag
Leu 302	-	expression tag
Ser 303	-	expression tag
Gly 304	-	expression tag
Leu 305	-	expression tag
Ser 306	-	expression tag
Gly 307	-	expression tag
Glu 308	-	expression tag
Gln 309	-	expression tag
Gly 310	-	expression tag
Pro 311	-	expression tag
Ser 312	-	expression tag
Gly 313	-	expression tag
Asp 314	-	expression tag
Met 315	-	expression tag
Thr 316	-	expression tag
Thr 317	-	expression tag
Glu 318	-	expression tag
Glu 319	-	expression tag
Asp 320	-	expression tag
Ser 321	-	expression tag
Ala 322	-	expression tag
Thr 323	-	expression tag
His 324	-	expression tag
Ile 325	-	expression tag
Lys 326	-	expression tag
Phe 327	-	expression tag
Ser 328	-	expression tag
Lys 329	-	expression tag
Arg 330	-	expression tag
Asp 331	-	expression tag
Glu 332	-	expression tag
Asp 333	-	expression tag
Gly 334	-	expression tag
Arg 335	-	expression tag
Glu 336	-	expression tag
Leu 337	-	expression tag
Ala 338	-	expression tag
Gly 339	-	expression tag
Ala 340	-	expression tag
Thr 341	-	expression tag
Met 342	-	expression tag
Glu 343	-	expression tag
Leu 344	-	expression tag
Arg 345	-	expression tag
Asp 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Lys 350	-	expression tag
Thr 351	-	expression tag
Ile 352	-	expression tag
Ser 353	-	expression tag
Thr 354	-	expression tag
Trp 355	-	expression tag
Ile 356	-	expression tag
Ser 357	-	expression tag
Asp 358	-	expression tag
Gly 359	-	expression tag
His 360	-	expression tag
Val 361	-	expression tag
Lys 362	-	expression tag
Asp 363	-	expression tag
Phe 364	-	expression tag
Tyr 365	-	expression tag
Leu 366	-	expression tag
Tyr 367	-	expression tag
Pro 368	-	expression tag
Gly 369	-	expression tag
Lys 370	-	expression tag
Tyr 371	-	expression tag
Thr 372	-	expression tag
Phe 373	-	expression tag
Val 374	-	expression tag
Glu 375	-	expression tag
Thr 376	-	expression tag
Ala 377	-	expression tag
Ala 378	-	expression tag
Pro 379	-	expression tag
Asp 380	-	expression tag
Gly 381	-	expression tag
Tyr 382	-	expression tag
Glu 383	-	expression tag
Val 384	-	expression tag
Ala 385	-	expression tag
Thr 386	-	expression tag
Pro 387	-	expression tag
Ile 388	-	expression tag
Glu 389	-	expression tag
Phe 390	-	expression tag
Thr 391	-	expression tag
Val 392	-	expression tag
Asn 393	-	expression tag
Glu 394	-	expression tag
Asp 395	-	expression tag
Gly 396	-	expression tag
Gln 397	-	expression tag
Val 398	-	expression tag
Thr 399	-	expression tag
Val 400	-	expression tag
Asp 401	-	expression tag
Gly 402	-	expression tag
Glu 403	-	expression tag
Ala 404	-	expression tag
Thr 405	-	expression tag
Glu 406	-	expression tag
Gly 407	-	expression tag
Asp 408	-	expression tag
Ala 409	-	expression tag
His 410	-	expression tag
Thr 411	-	expression tag
Gly 412	-	expression tag
Gly 413	-	expression tag
Ser 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag

H, I, J, K, L, M, N: 1 - 293 (UniProt: P09616)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 122	Gly 148	engineered mutation
Arg 147	Lys 173	engineered mutation
Gly 294	-	expression tag
Ser 295	-	expression tag
Ser 296	-	expression tag
Gly 297	-	expression tag
Ser 298	-	expression tag
Arg 299	-	expression tag
Gly 300	-	expression tag
Val 301	-	expression tag
Pro 302	-	expression tag
His 303	-	expression tag
Ile 304	-	expression tag
Val 305	-	expression tag
Met 306	-	expression tag
Val 307	-	expression tag
Asp 308	-	expression tag
Ala 309	-	expression tag
Tyr 310	-	expression tag
Lys 311	-	expression tag
Arg 312	-	expression tag
Tyr 313	-	expression tag
Lys 314	-	expression tag
Gly 315	-	expression tag
Gly 316	-	expression tag
Ser 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	586652.1
All*	Total formula weight	586652.1

*Water molecules are not included.