8JPZ

The thermostability mutant Gox_M8 from Aspergillus niger

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glucose oxidase (Fragment)	polymer	581	63154.4	2	UniProt (Q5Q041) Pfam (PF00732) Pfam (PF05199) In PDB	Aspergillus niger
2	C, D	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
3	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
4	A, B	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
5	A, B	OXYGEN MOLECULE	non-polymer		32.0	2	Chemie (OXY)
6	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	11	Chemie (NAG)
7	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
8		water	water		18.0	862	Chemie (HOH)

Sequence modifications

A, B: 1 - 581 (UniProt: Q5Q041)

PDB	External Database	Details
Val 31	Thr 31	conflict
Val 32	Thr 32	conflict
Lys 68	Asp 68	conflict
Cys 82	Glu 82	conflict
Arg 88	Gln 88	conflict
Ala 94	Ser 94	conflict
Phe 274	Thr 274	conflict
Thr 278	Tyr 278	conflict
Lys 313	Asp 313	conflict
Glu 418	Val 418	conflict
His 508	Asn 508	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	126308.8
Branched	Number of molecules	2
	Total formula weight	2145.9
Non-Polymers*	Number of molecules	18
	Total formula weight	4663.2
All*	Total formula weight	133117.9

*Water molecules are not included.