8JPZ

The thermostability mutant Gox_M8 from Aspergillus niger

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	126.450, 126.450, 193.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.180 - 2.080
R-factor	0.1674
Rwork	0.166
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.180	2.154
High resolution limit [Å]	2.080	2.080
Number of reflections	94279	9278
<I/σ(I)>	8.86	1.21
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.997	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M sodium chloride, 0.1 M HEPES buffer (pH 7.0), and 12% (w/v) polyethylene glycol monomethyl ether 2000