8JPP

Cryo-EM structure of succinate receptor bound to succinate acid coupling MiniGsq

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	253	29245.1	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Soluble cytochrome b562,Succinate receptor 1,Oplophorus-luciferin 2-monooxygenase catalytic subunit	polymer	658	74993.5	1	UniProt (P0ABE7) UniProt (Q9BXA5) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,G-protein coupled receptor 91,P2Y purinoceptor 1-like
5	E (R)	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)

Sequence modifications

A: 1 - 384 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict
Gly 196	-	insertion
Gly 197	Val 65	conflict
Ser 198	Asn 66	conflict
Gly 200	Phe 68	conflict
Ser 201	Asn 69	conflict
-	Asp 71	deletion
-	Ser 72	deletion
-	Glu 73	deletion
-	Lys 74	deletion
-	Ala 75	deletion
-	Thr 76	deletion
-	Lys 77	deletion
-	Val 78	deletion
-	Gln 79	deletion
-	Asp 80	deletion
-	Ile 81	deletion
-	Lys 82	deletion
-	Asn 83	deletion
-	Asn 84	deletion
-	Leu 85	deletion
-	Lys 86	deletion
-	Glu 87	deletion
-	Ala 88	deletion
-	Ile 89	deletion
-	Glu 90	deletion
-	Thr 91	deletion
-	Ile 92	deletion
-	Val 93	deletion
-	Ala 94	deletion
-	Ala 95	deletion
-	Met 96	deletion
-	Ser 97	deletion
-	Asn 98	deletion
-	Leu 99	deletion
-	Val 100	deletion
-	Pro 101	deletion
-	Pro 102	deletion
-	Val 103	deletion
-	Glu 104	deletion
-	Leu 105	deletion
-	Ala 106	deletion
-	Asn 107	deletion
-	Pro 108	deletion
-	Glu 109	deletion
-	Asn 110	deletion
-	Gln 111	deletion
-	Phe 112	deletion
-	Arg 113	deletion
-	Val 114	deletion
-	Asp 115	deletion
-	Tyr 116	deletion
-	Ile 117	deletion
-	Leu 118	deletion
-	Ser 119	deletion
-	Val 120	deletion
-	Met 121	deletion
-	Asn 122	deletion
-	Val 123	deletion
-	Pro 124	deletion
-	Asp 125	deletion
-	Phe 126	deletion
-	Asp 127	deletion
-	Phe 128	deletion
-	Pro 129	deletion
-	Pro 130	deletion
-	Glu 131	deletion
-	Phe 132	deletion
-	Tyr 133	deletion
-	Glu 134	deletion
-	His 135	deletion
-	Ala 136	deletion
-	Lys 137	deletion
-	Ala 138	deletion
-	Leu 139	deletion
-	Trp 140	deletion
-	Glu 141	deletion
-	Asp 142	deletion
-	Glu 143	deletion
-	Gly 144	deletion
-	Val 145	deletion
-	Arg 146	deletion
-	Ala 147	deletion
-	Cys 148	deletion
-	Tyr 149	deletion
-	Glu 150	deletion
-	Arg 151	deletion
-	Ser 152	deletion
-	Asn 153	deletion
-	Glu 154	deletion
-	Tyr 155	deletion
-	Gln 156	deletion
-	Leu 157	deletion
-	Ile 158	deletion
-	Asp 159	deletion
-	Cys 160	deletion
-	Ala 161	deletion
-	Gln 162	deletion
-	Tyr 163	deletion
-	Phe 164	deletion
-	Leu 165	deletion
-	Asp 166	deletion
-	Lys 167	deletion
-	Ile 168	deletion
-	Asp 169	deletion
-	Val 170	deletion
-	Ile 171	deletion
-	Lys 172	deletion
-	Gln 173	deletion
-	Ala 174	deletion
-	Asp 175	deletion
-	Tyr 176	deletion
-	Val 177	deletion
-	Pro 178	deletion
-	Ser 179	deletion
-	Asp 180	deletion
-	Gln 181	deletion
-	Asp 182	deletion
-	Leu 183	deletion
-	Leu 184	deletion
-	Arg 185	deletion
-	Cys 186	deletion
-	Arg 187	deletion
-	Val 188	deletion
Gly 203	Leu 189	conflict
Asp 249	Ala 235	conflict
Asp 252	Ser 238	conflict
-	Asn 240	deletion
-	Met 241	deletion
-	Val 242	deletion
-	Ile 243	deletion
-	Arg 244	deletion
-	Glu 245	deletion
-	Asp 246	deletion
-	Asn 247	deletion
-	Gln 248	deletion
-	Thr 249	deletion
Ala 362	Ile 358	conflict
Ile 365	Val 361	conflict
Lys 370	Arg 366	conflict
Leu 374	Gln 370	conflict
Gln 375	Arg 371	conflict
Asn 377	His 373	conflict
Glu 380	Gln 376	conflict
Asn 382	Glu 378	conflict
Val 384	Leu 380	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Met -162	-	initiating methionine
Lys -161	-	expression tag
Thr -160	-	expression tag
Ile -159	-	expression tag
Ile -158	-	expression tag
Ala -157	-	expression tag
Leu -156	-	expression tag
Ser -155	-	expression tag
Tyr -154	-	expression tag
Ile -153	-	expression tag
Phe -152	-	expression tag
Cys -151	-	expression tag
Leu -150	-	expression tag
Val -149	-	expression tag
Phe -148	-	expression tag
Ala -147	-	expression tag
Asp -146	-	expression tag
Tyr -145	-	expression tag
Lys -144	-	expression tag
Asp -143	-	expression tag
Asp -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Ala -139	-	expression tag
Gly -138	-	expression tag
Arg -137	-	expression tag
Ala -136	-	expression tag
His -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
His -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
Glu -125	-	expression tag
Asn -124	-	expression tag
Leu -123	-	expression tag
Tyr -122	-	expression tag
Phe -121	-	expression tag
Gln -120	-	expression tag
Ser -119	-	expression tag
Gly -118	-	expression tag
Ala -117	-	expression tag
Pro -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Leu -9	-	linker
Glu -8	-	linker
Val -7	-	linker
Leu -6	-	linker
Phe -5	-	linker
Gln -4	-	linker
Gly -3	-	linker
Pro -2	-	linker
Glu -1	-	linker
Phe 0	-	linker

R: 1 - 334 (UniProt: Q9BXA5)

PDB	External Database	Details
Ala 335	-	linker
Ala 336	-	linker
Ala 337	-	linker
Val 338	-	linker

R: 339 - 495 (PDB: 8JPP)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	153155.6
Non-Polymers*	Number of molecules	1
	Total formula weight	118.1
All*	Total formula weight	153273.7

*Water molecules are not included.