8JPC
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | L | NTS(8-13) | polymer | 6 | 819.0 | 1 | synthetic construct | ||
| 2 | R | Neurotensin receptor type 1 | polymer | 418 | 46307.6 | 1 | UniProt (P30989) Pfam (PF00001) In PDB | Homo sapiens (human) | NT-R-1,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH |
| 3 | G | Beta-adrenergic receptor kinase 1 | polymer | 688 | 79644.7 | 1 | UniProt (P21146) Pfam (PF00615) Pfam (PF00069) Pfam (PF00169) In PDB | Bos taurus (cattle) | Beta-ARK-1,G-protein-coupled receptor kinase 2 |
| 4 | Q | Guanine nucleotide-binding protein G(q) subunit alpha | polymer | 353 | 41282.9 | 1 | UniProt (P50148) Pfam (PF00503) In PDB | Homo sapiens (human) | Guanine nucleotide-binding protein alpha-q |
| 5 | R | 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol | non-polymer | 450.5 | 1 | Chemie (SRW) | |||
| 6 | G | STAUROSPORINE | non-polymer | 466.5 | 1 | Chemie (STU) | |||
| 7 | Q | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 8 | Q | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | Q | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) |
Sequence modifications
G: 2 - 689 (UniProt: P21146)
Q: 37 - 359 (UniProt: P50148)
| PDB | External Database | Details |
|---|---|---|
| Pro 292 | Ala 292 | engineered mutation |
| Ile 295 | Arg 295 | engineered mutation |
| Asp 455 | Ser 455 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 7 | - | initiating methionine |
| Gly 8 | - | expression tag |
| Cys 9 | - | expression tag |
| Thr 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Ser 12 | - | expression tag |
| Ala 13 | - | expression tag |
| Glu 14 | - | expression tag |
| Asp 15 | - | expression tag |
| Lys 16 | - | expression tag |
| Ala 17 | - | expression tag |
| Ala 18 | - | expression tag |
| Val 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Arg 21 | - | expression tag |
| Ser 22 | - | expression tag |
| Lys 23 | - | expression tag |
| Met 24 | - | expression tag |
| Ile 25 | - | expression tag |
| Asp 26 | - | expression tag |
| Arg 27 | - | expression tag |
| Asn 28 | - | expression tag |
| Leu 29 | - | expression tag |
| Arg 30 | - | expression tag |
| Glu 31 | - | expression tag |
| Asp 32 | - | expression tag |
| Gly 33 | - | expression tag |
| Glu 34 | - | expression tag |
| Arg 35 | - | expression tag |
| Ser 36 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 168054.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1487.6 | |
| All* | Total formula weight | 169541.8 |
