8JP6

Cryo-EM structures of the head region of full-length ERGIC-53 with MCFD2 (Substate A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	Protein ERGIC-53	polymer	522	58766.0	4	UniProt (P49257) Pfam (PF03388)	Homo sapiens (human)	ER-Golgi intermediate compartment 53 kDa protein,Gp58,Intracellular mannose-specific lectin MR60,Lectin mannose-binding 1
2	C, D, G, H (C, D, G, H)	Multiple coagulation factor deficiency protein 2	polymer	124	13952.2	4	UniProt (Q8NI22) Pfam (PF13499)	Homo sapiens (human)	Neural stem cell-derived neuronal survival protein
3	AA, I, J, K, L... (H, A, B, C, D...)	CALCIUM ION	non-polymer		40.1	16	Chemie (CA)
4	BA, O, R, Y (H, C, D, G)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, B, E, F: 1 - 510 (UniProt: P49257)

PDB	External Database	Details
Gly 511	-	expression tag
Val 512	-	expression tag
Ala 513	-	expression tag
Met 514	-	expression tag
Pro 515	-	expression tag
Gly 516	-	expression tag
Ala 517	-	expression tag
Glu 518	-	expression tag
Asp 519	-	expression tag
Asp 520	-	expression tag
Val 521	-	expression tag
Val 522	-	expression tag

C, D, G, H: 27 - 146 (UniProt: Q8NI22)

PDB	External Database	Details
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	290873.0
Non-Polymers*	Number of molecules	20
	Total formula weight	902.9
All*	Total formula weight	291775.9

*Water molecules are not included.