8JP6

Cryo-EM structures of the head region of full-length ERGIC-53 with MCFD2 (Substate A)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016020	cellular_component	membrane
B	0016020	cellular_component	membrane
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005793	cellular_component	endoplasmic reticulum-Golgi intermediate compartment
C	0005794	cellular_component	Golgi apparatus
C	0010467	biological_process	gene expression
C	0012507	cellular_component	ER to Golgi transport vesicle membrane
C	0015031	biological_process	protein transport
C	0016192	biological_process	vesicle-mediated transport
C	0033116	cellular_component	endoplasmic reticulum-Golgi intermediate compartment membrane
C	0046872	molecular_function	metal ion binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005793	cellular_component	endoplasmic reticulum-Golgi intermediate compartment
D	0005794	cellular_component	Golgi apparatus
D	0010467	biological_process	gene expression
D	0012507	cellular_component	ER to Golgi transport vesicle membrane
D	0015031	biological_process	protein transport
D	0016192	biological_process	vesicle-mediated transport
D	0033116	cellular_component	endoplasmic reticulum-Golgi intermediate compartment membrane
D	0046872	molecular_function	metal ion binding
E	0016020	cellular_component	membrane
F	0016020	cellular_component	membrane
G	0005509	molecular_function	calcium ion binding
G	0005515	molecular_function	protein binding
G	0005783	cellular_component	endoplasmic reticulum
G	0005789	cellular_component	endoplasmic reticulum membrane
G	0005793	cellular_component	endoplasmic reticulum-Golgi intermediate compartment
G	0005794	cellular_component	Golgi apparatus
G	0010467	biological_process	gene expression
G	0012507	cellular_component	ER to Golgi transport vesicle membrane
G	0015031	biological_process	protein transport
G	0016192	biological_process	vesicle-mediated transport
G	0033116	cellular_component	endoplasmic reticulum-Golgi intermediate compartment membrane
G	0046872	molecular_function	metal ion binding
H	0005509	molecular_function	calcium ion binding
H	0005515	molecular_function	protein binding
H	0005783	cellular_component	endoplasmic reticulum
H	0005789	cellular_component	endoplasmic reticulum membrane
H	0005793	cellular_component	endoplasmic reticulum-Golgi intermediate compartment
H	0005794	cellular_component	Golgi apparatus
H	0010467	biological_process	gene expression
H	0012507	cellular_component	ER to Golgi transport vesicle membrane
H	0015031	biological_process	protein transport
H	0016192	biological_process	vesicle-mediated transport
H	0033116	cellular_component	endoplasmic reticulum-Golgi intermediate compartment membrane
H	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DYDGNNLLDglEL

Chain	Residue	Details
C	ASP81-LEU93
C	ASP129-PHE141

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	36
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448

Chain	Residue	Details
C	ASP81
D	ASP81
D	ASP83
D	ASN85
D	GLU92
D	ASP129
D	ASN131
D	ASP133
D	TYR135
D	GLU140
G	ASP81
C	ASP83
G	ASP83
G	ASN85
G	GLU92
G	ASP129
G	ASN131
G	ASP133
G	TYR135
G	GLU140
H	ASP81
H	ASP83
C	ASN85
H	ASN85
H	GLU92
H	ASP129
H	ASN131
H	ASP133
H	TYR135
H	GLU140
C	GLU92
C	ASP129
C	ASN131
C	ASP133
C	TYR135
C	GLU140

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8K5B3

Chain	Residue	Details
C	SER106
D	SER106
G	SER106
H	SER106

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site_id	SWS_FT_FI3
Number of Residues	44
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
A	ARG499-PHE510
B	ARG499-PHE510
E	ARG499-PHE510
F	ARG499-PHE510

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00658

Chain	Residue	Details
A	SER88
E	ASP121
E	HIS178
E	GLY251
F	SER88
F	ASP121
F	HIS178
F	GLY251
A	ASP121
A	HIS178
A	GLY251
B	SER88
B	ASP121
B	HIS178
B	GLY251
E	SER88

---

site_id	SWS_FT_FI5
Number of Residues	16
Details	BINDING:

Chain	Residue	Details
A	ASP152
E	PHE154
E	ASN156
E	ASP181
F	ASP152
F	PHE154
F	ASN156
F	ASP181
A	PHE154
A	ASN156
A	ASP181
B	ASP152
B	PHE154
B	ASN156
B	ASP181
E	ASP152

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	SITE: Required for ER export

Chain	Residue	Details
A	GLN501
B	GLN501
E	GLN501
F	GLN501

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER425
B	SER425
E	SER425
F	SER425

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