8JNK

Crystal structure of human ALKBH3 bound to ssDNA through active site crosslink

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3	polymer	232	27013.3	4	UniProt (Q96Q83) Pfam (PF13532)	Homo sapiens (human)	Alkylated DNA repair protein alkB homolog 3,hABH3
2	B, D, F, H (B, D, F, H)	DNA	polymer	6	1844.3	4		Homo sapiens
3	I, J, K, L (I, J, K, L)	Synthetic antibody heavy chain	polymer	216	22927.5	4		Homo sapiens (human)
4	M, N, O, P (M, N, O, P)	Synthetic antibody light chain	polymer	217	23667.3	4		Homo sapiens (human)
5	Q, S, U, W (A, C, E, G)	N-OXALYLGLYCINE	non-polymer		147.1	4	Chemie (OGA)
6	R, T, V, X (A, C, E, G)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
7	AA, BA, CA, DA, EA... (C, E, F, G, H...)	water	water		18.0	277	Chemie (HOH)

Sequence modifications

A, C, E, G: 70 - 280 (UniProt: Q96Q83)

PDB	External Database	Details
Met 49	-	initiating methionine
Gly 50	-	expression tag
Ser 51	-	expression tag
Ser 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag
His 58	-	expression tag
Ser 59	-	expression tag
Ser 60	-	expression tag
Gly 61	-	expression tag
Leu 62	-	expression tag
Val 63	-	expression tag
Pro 64	-	expression tag
Arg 65	-	expression tag
Gly 66	-	expression tag
Ser 67	-	expression tag
His 68	-	expression tag
Met 69	-	expression tag
Ser 110	Cys 110	engineered mutation
Cys 195	Glu 195	conflict
Ser 201	Cys 201	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	301809.8
Non-Polymers*	Number of molecules	8
	Total formula weight	808.1
All*	Total formula weight	302617.9

*Water molecules are not included.