8JNK
Crystal structure of human ALKBH3 bound to ssDNA through active site crosslink
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9875 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.460, 136.151, 203.390 |
| Unit cell angles | 90.00, 107.11, 90.00 |
Refinement procedure
| Resolution | 46.900 - 2.690 |
| R-factor | 0.2182 |
| Rwork | 0.218 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.790 |
| High resolution limit [Å] | 2.690 | 5.790 | 2.690 |
| Rmerge | 0.077 | 0.028 | 0.688 |
| Total number of observations | 348882 | ||
| Number of reflections | 92893 | 9401 | 9152 |
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.3 | 98.7 | 98.5 |
| Redundancy | 3.8 | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M NaCl, 20%PEG6000 |
