8JIU
Cryo-EM structure of the GLP-1R/GCGR dual agonist SAR425899-bound human GCGR-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
2 | P | SAR425899 | polymer | 29 | 3243.6 | 1 | synthetic construct | ||
3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Rat) | Transducin beta chain 1 |
4 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (Bovine) | G gamma-I |
5 | N | Nanobody 35 | polymer | 140 | 15343.0 | 1 | Escherichia coli | ||
6 | R | Glucagon receptor | polymer | 405 | 46627.3 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) In PDB | Homo sapiens (human) | GL-R |
7 | P | N-hexadecanoyl-L-glutamic acid | non-polymer | 385.5 | 1 | Chemie (D6M) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
PDB | External Database | Details |
---|---|---|
Asn 54 | Ser 54 | conflict |
Ala 226 | Gly 226 | conflict |
Ala 268 | Glu 268 | conflict |
Lys 271 | Asn 271 | conflict |
Asp 274 | Lys 274 | conflict |
Lys 280 | Arg 280 | variant |
Asp 284 | Thr 284 | conflict |
Thr 285 | Ile 285 | conflict |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 156542.7 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 385.5 | |
All* | Total formula weight | 156928.3 |